Adsorption immobilization of biomolecules from subphase on Langmuir monolayers of organo-modified single-walled carbon nanotube

The subphase adsorption behavior and the morphology of trypsin enzyme, lysozyme cytochrome C protein, glucose oxidase present on Langmuir monolayer organo-modified single-wall carbon nanotubes (SWCNTs) were investigated. Furthermore, we estimated maintenance characteristics three-dimensional structure SWCNT film-adsorbed biomolecules when exposed to a high-temperature environment. In this study, each biomolecule, which was an amphoteric electrolyte, hydrophilized by mixed acid treatment electrostatically adsorbed charged surfaces at air–water interface. An amide band derived from adsorbent confirmed infrared (IR) spectra biomolecule adsorbing organo-SWCNT multilayers. atomic force microscopic image X-type organo-SWCNTs shows shape. out-of-plane X-ray diffraction profiles multilayers layered due presence adsorbent. After heat treatment, secondary biomolecular maintained up 100–150 °C, as IR spectrum.